发明名称 |
Method for production of lymphotoxin (TNFB) in cell line A-C5-8 |
摘要 |
Lymphotoxin-producing human T-cell hybridomas are incubated in a medium containing phorbol myristate acetate, concanavalin-A or a mixture thereof, the resulting cells are fractionated by sucrose density-gradient centrifugation method to isolate a messenger RNA in 12.6S to 14.6S fractions, and a gene containing a part encoding a polypeptide having a lymphotoxin-activity is prepared from the messenger RNA. The gene is represented by the nucleotide sequence of the Table 1 as herein given. Using the present gene, a new lymphotoxin having the amino acid sequence (I) or (II) as given herein can be obtained by genetic engineering technology.
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申请公布号 |
US5403725(A) |
申请公布日期 |
1995.04.04 |
申请号 |
US19910733974 |
申请日期 |
1991.07.22 |
申请人 |
DENKI KAGAKU KOGYO KABUSHIKI KAISHA |
发明人 |
OSAWA, TOSHIAKI;OBINATA, MASUO;ISHII, YOSHIYUKI;KOBAYASHI, YOSHIO |
分类号 |
A61K38/00;C07K14/525;(IPC1-7):A61K45/05;C12P21/06;C12N15/00 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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