| 摘要 |
PURPOSE:To provide the subject gene coding human alpha1C adrenalin receptor, facilitating researches for clarifying the function of the receptor, useful for the retrieval of agonists or antagonists for the receptor, and capable of producing the adrenalin receptor. CONSTITUTION:The cDNA library of a human prostate is first screened to select a positive clone, while using as probes the fragment of a part highly homologous with a human alpha1 adrenalin receptor family in the gene of a human cerebral alpha1A adrenalin receptor and the PCR product of a bovine alpha1C adrenalin receptor. A DNA is recovered from the positive clone, and subsequently treated with a restriction enzyme to obtain a gene coding the human alpha1C adrenalin receptor. The gene can be utilized to obtain a sufficient amount of the purified human alpha1C adrenalin receptor by a recombined DNA technique comprising ligating the gene to a vector and subsequently transforming a host with the vector, and can be used for researched for clarifying the function of the gene, for the retrievals of agonists or antagonists of the receptor, etc. |
