| 摘要 |
<p>Particles and methods for the detection or visualization of analytes, including nucleic acids, using fluorescence energy transfer or intramolecular energy transfer. Particles comprising an energy donor as a first component and a fluorescent dye as a second component positioned in said particles at an energy exchanging distance from one another, wherein the two components have a Stockes shift of greater than or equal to 50 nm, said particle having bound on its surface, a protein, polypeptide, nucleic acid, nucleotide or protein containing ligand analogue are disclosed and claimed. In addition, novel fluorescent dyes are described which exhibit intramolecular energy transfer for use to label various molecules, proteins, polypeptides, nucleotides and nucleic acids or to incorporate into particles. Many novel phthalocyanine derivatives and hybrid phthalocyanine derivatives are disclosed and claimed. Such derivatives also may contain an electron transfer subunit. Axial ligands may be covalently bound to the metals contained in the hybrid phthalocyanine derivatives. Numerous compounds capable of intramolecular energy transfer as well as compounds for fluorescence energy transfer are claimed.</p> |
