Human interferon-beta 2A and interfeon-beta 2B, vectors containing genes coding for said interferons, cell lines producing same and use of said interferons as pharmaceuticals.

摘要

Human interferon-beta2A and interferon-beta2B are produced in purified form by recombinant DNA techniques. Two separate human genes have been identified which code for the production of IFN- beta 2A and IFN- beta 2B, respectively. The sequence of IFN- beta 2A cDNA is established. These genes and cDNA have been cloned into mammalian cells with an SV40 early promoter sequence and such genomic clones are capable of producing IFN- beta 2A and IFN- beta 2B. The antiviral activity of such recombinant IFN- beta 2A and IFN- beta 2B is demonstrated as well as other biological activity identifying them as human interferons. It has been shown that IFN- beta 2 secretion is induced in human cells by growth-stimulatory cytokines. IFN- beta 2A may be used for preparing pharmaceutical compositions useful in the fields of inflammation,, acute phase response or regulation of cell proliferation. Further uses are suggested.