| 摘要 |
The present invention relates to new Bacillus sp. SY-414 that produced antifungal antibiotic, trihexocin, which was produced by the above Bacillus sp. SY-414 and its manufacturing method. New antifungal antibiotic represented by general formula (1), wherein each R denotes -H, -OH, -NH27 alkyl group of C1-C6, allyl group of C5C20, or alkylene group of C2-C6, and subscript l, m, or n is an integer less than 3. The manufacturing method for the above antifungal antibiotic is as follows: new Bacillus sp. microorganism showing a high activity against plant fungi, especially Pyricularia oryzae and Pellicularia filamentosa, was isolated from soil and identified; the culture broth was centrifuged and the supernatant was extracted with ethyl acetate, and the organic layer was collected. The active fraction was evaporated and dissolved in methanol, and then silica gel column chromatography was carried out; the active fraction was evaporated and dissolved in dichloromethane: methanol (1:5) mixture and Sephadex LH-20 column chromatography was carried out. The active fraction was evaporated and dissolved in water and lyophilized. The powdery antibiotic obtained in the above was dissolved in methanol and stored at 4 .degree.C, and the crystalline antibiotic, trihexocin, was prepared; trihexocin had an antifungal activity against the plants infected with fungi at the concentration of 50-100 .mu.g/ml. Tihexocin consisted of three fructose units and showed a non-toxicity.
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