| 摘要 |
<p>This invention relates to a reagent for enzymatic determination of the concentration of serum bicarbonate levels in a patient wherein the degree of oxidation of a coenzyme is measured, characterised in that said reagent is stabilised against oxidation by a coenzyme reduction system comprising an enzyme and substrate pair selected so as to enable continuous regeneration of said coenzyme throughout storage of said reagent. Also disclosed is an improvement in an enzymatic method of determination of the concentration of serum bicarbonate in a sample body fluid wherein the degree of oxidation of a coenzyme is measured, the improvement comprising stabilizing a reagent comprising said coenzyme against oxidation by a coenzyme reduction system comprising an enzyme and substrate pair selected so as to enable continuous regeneration of said coenzyme throughout storage of said reagent.</p> |
