| 摘要 |
A method is provided for producing adipic acid. The method comprises the steps of culturing a cell transformant capable of converting a carbon source to catechol for a period of time sufficient to convert said carbon source to catechol, biocatalytically converting the catechol to cis, cis-muconic acid using catechol 1,2-dioxygenase, and hydrogenating the cis, cis-muconic acid to produce adipic acid. Also provided is a heterologous transformant of the host cell having an endangeous common pathway of aromatic amino acid biosynthesis. The heterologous transformant is characterized by the constitutive expression of structural genes encoding 3-dehydroshikimate dehydratase, protocatechuate decarboxylase, and catechol 1,2-dioxygenase. |
