METHOD FOR EXPRESSION OF GENE USING HEAT SHOCK PROMOTER AND USE THEREOF

摘要

A bacterial strain, for example Escherichia coli, contains and replicates a recombinant DNA plasmid comprising a vector, a fragment of plant DNA controlling expression of a heat shock gene from an Agrobacterium strain inserted into a T-DNA fragment of a Ti-plasmid and a foreign gene or a soybean gene under control of said fragment of plant DNA. A suitable vector is pBR322, and examples of suitable foreign genes include a Z-gene coating for beta -galactosidase or a crystalline endotoxin gene. Also disclosed are methods for recognising plant cells transformed by T-DNA of Agrobacterium species by expressing a transformation recognition gene under control of a soybean heat shock gene promoter.