| 摘要 |
PURPOSE:To obtain the novel protease useful as an active component of, e.g. a preventive and. therapeutic agent for hepatic diseases, an activating and preventive agent for an injured nerve cell or a preventive and therapeutic agent for AIDS from a strain belonging to Streptomyces. CONSTITUTION:A novel metalloend peptidase having the following physicochemical properties; action: having an action on milk casein, egg white albumin, bovine serum albumin, bradykinin, angiotensin II, etc.; substrate specificity: having a specific activity on the amide bond part of carbobenzoxyglycylleucinamide and carbobenzoxy-glycylphenylalaninamide as a synthetic substrate, an pyro-glutamylphenylalanylleucin-para-nitroanilide bond part and a leucine-para-nitroanilide bond part; optimum pH: 8.5 to 9.0; optimum temperature: 65 to 70 deg.C; stable pH range: 3.0 to 9.5; stable temperature range: 65 to 80 deg.C. This enzyme can be obtained by culturing a strain belonging to Streotomyces and capable of producing metalloend peptidase F. |
