PRODUCTION METHOD FOR PREPARATION OF DISABLED VIRUSES

摘要

A transgenic species whose genome comprises a heterologous gene which is functionally equivalent to a non-retroviral virus gene which is essential for the production of normal infectious non-retroviral virus is taught. A method for manufacturing a mutant virus whose genome is disabled in respect of at least one virus gene essential for the production of infectious virus, such that the virus can infect normal cells and undergo replication and expression of viral antigen gene in those cells, but cannot produce normal infectious virus, which method comprises following the production of a transgenic animal whose genome comprises at least one heterologous gene which is functionally equivalent to the at least one virus gene, infecting at least one cell derived from the animal which contains the at least one heterologous gene with mutant virus; causing the at least one cell to express the heterologous gene so as to allow production of infectious virus particles containing the disabled gene; and harvesting infectious virus particles containing the disabled genome.