REAGENT AND METHOD OF ITS USE

摘要

Reagent composition systems useful in an oscillating particle type coagulation assay in which a liquid blood or plasma specimen is moved to an assay location in an assay cartridge (1) and held at that location during the assay by capillary forces. A liquid specimen such as blood is applied to sample port (1), drawn by capillary forces in passageway (3) and, moved to assay site (4). Vent port (5) is open to the atmosphere. A first reagent layer (6) includes magnetizable particles dispersed in an aqueous solution of carbohydrates of both higher and lower molecular weights selected so as to encourage coating of the reagent on a surface from a solution and easy resolubilization of the coated material. Reagent layer (7) is placed on reagent layer (6) at assay site (4). Reagent layer (7) comprises a mixture of higher and lower molecular weight carbohydrates mixed with an aqueous thromboplastin reagent.