Process for the preparation of heterologous bispecific antibodies
摘要
The invention pertains to a method for producing heterologous bispecific antibodies. The object of the invention is a method designed to yield heterologous bispecific antibodies with a high level of purity. This is attained by producing a quadroma fused from hybridomas, one of which produces antibodies that have an affinity to the binding site of protein A and the other of which produces antibodies that have a weaker or no affinity to the binding site of protein A; by propagating and cultivating the quadromas as usual, and by eluting the bsAk in a pH range at least 0.5 units above the pH at which the antibodies with greater affinity to the binding site of protein A are still bonded.
申请公布号
DE4419399(C1)
申请公布日期
1995.03.09
申请号
DE19944419399
申请日期
1994.06.03
申请人
GSF - FORSCHUNGSZENTRUM FUER UMWELT UND GESUNDHEIT GMBH, 85764 OBERSCHLEISHEIM, DE
发明人
LINDHOFER, HORST, DR., 80999 MUENCHEN, DE;THIERFELDER, STEFAN, PROF. DR., 82223 EICHENAU, DE