Process for extraction of nucleic acids and method for detecting specified nucleic acid sequences

摘要

The invention provides a process for extracting nucleic acids from a sample, which comprises the following steps: - mixing the sample with a carrier which is at least one of the substances dextran, acrylamide or carboxymethylcellulose in order to produce a liquid mixture; - mixing the liquid mixture with a reagent C in order to precipitate the nucleic acids and the carrier, where the reagent C contains at least one reagent A selected from the substances guanidinium thiocyanate, guanidinium hydrochloride, potassium thiocyanate and sodium thiocyanate, and at least one reagent B selected from the substances n-propyl alcohol, isopropyl alcohol, n-butyl alcohol, sec-butyl alcohol, tert-butyl alcohol and tert-amyl alcohol; and - separating the precipitated nucleic acids and the precipitated carrier from the liquid phase. The process is easier to use than the procedures hitherto practised, it contains a smaller number of steps, is able to reduce the possibility of aerosol formation, can be carried out within a short time, requires no phenol or chloroform and, nevertheless, achieves a constant efficiency of extraction of nucleic acids. Also disclosed is a method for detecting a particular nucleic acid sequence.