PROCEDE DE CULTURE EN SERIE DE KERATINOCYTES

摘要

A method is disclosed for serially culturing keratinocytes such as human epidermal cells and teratomal keratinocytes. These can be serially grown in culture to produce colonies and eventually stratified squamous epithelium by including in the culture fibroblast cells or medium harvested from fibroblast cultures. When fibroblast cells are used, they are treated to prevent their multiplication, and they are also introduced into the cultures at a controlled density.