COMPOSITIONS AND METHODS FOR DETECTING TARGET NUCLEIC ACID SEQUENCES UTILIZING FLANKING SEQUENCE ENZYME MOLECULES

摘要

Briefly stated, the present invention provides novel compositions and methods for detecting target nucleic acid sequences utilizing adjacent sequence-enzyme molecules. Within one aspect of the present invention, oligonucleotide-enzyme fusion molecules are provided, comprising an enzyme capable of cleaving scissile linkages and an oligonucleotide having the structure (NA1)x-S2-(NA2)y wherein NA1 and NA2 are nucleic acid sequences, S is a scissile nucleic acid linkage, x, y, and z are integers from 1 to 1,000 and n is an integer from 1 to 10.