| 摘要 |
<p>Porcine embryos are cryopreserved using a method for reducing the level of lipid within cells of the embryo followed by freezing the embryo. Reduction of the level of lipid can be achieved either by polarizing the lipid within the embryo by centrifugation of the embryo, followed by rapid freezing or by microsurgical removal of the polarized lipid. Alternatively the level of lipid may be reduced by culturing the embryo in vitro. The reduction of the level of lipid before freezing results in good survival rates and can be used on zona intact porcine embryos.</p> |
