IN SITU EXTRACTION OF MICROBIAL DNA

摘要

A method for the extraction of microbial DNA which renders such DNA amenable for further enzymatic modification in situ, including digestion of the DNA with restriction endonuclease. The method comprises: a) heating the microbial cells in the presence of a hypotonic EDTA solution, b) lysing the cells by addition of achromopeptidase and c) heating said lysed cells to inactivate remaining protease and nuclease.