TEMPLATE ASSEMBLED SYNTHETIC PROTEIN

摘要

A chemoselective ligation approach to the preparation of complex peptides is employed in a simple, convenient synthesis of templateassembled synthetic protein (TASP) molecules. Reaction of readily prepared synthetic pro-helical peptide-.alpha.COSH molecules, in unprotected form, with a synthetic (BrAc)4template peptide molecule, also in unprotected form, proceeds rapidly in aqueous solution to give a uniform product in high yield. The resulting TASP molecule may be simply purified to homogeneity. Structural homogeneity may be demonstrated by ionspray mass spectrometry. The chemoselective ligation of unprotected peptides represents a general approach to the synthesis of TASP molecules.