CDNA probes and antibodies for human methenyltetrahydrofolate synthetase

摘要

The present invention relates to a cDNA probe for the detection of the mRNA for human methenyltetrahydrofolate synthetase, which comprises the following nucleic acid sequence, Seq. Id. No. 1: GGCCAAC -1 ATGGTGAAACCCCATCTCTATCAG AAATACAAAAGAATTTCCATC 45 TTTCTGAGCATGCAAGATGAAATT GAGACAGAAGAGATCATCAAG 90 GACATTTTCCAACGAGGCAAAATC TGCTTCATCCCTCGGTACCGG 135 TTCCAGAGCAATCACATG GATATG GTGA GAATAGAATCACCA GA G 180 GAAATTTCTTTACTTCCCAAAACA TCCTGGAATATCCCTCAGCCT 225 GGTGAGGGTGATGTTCGGGAGGAG GCCTTGTCCACAGGGGGACTT 270 GATCTCATCTTCATGCCAGGCCTT GGGTTTGACAAACATGGCAAC 315 CGACTGGGGAGGGGCAAGGGCTAC TATGATGCCTATCTGAAGCGC 360 TGTTTGCAGCATCAGGAAGTGAAG CCCTACACCCTGGCGTTGGCT 405 TTCAAAGAACAGATTTGCCTCCAG GTCCCAGTGAATGAAAACGAC 450 ATGAAGGTAGATGAAGTCCTTT 472 or any functional analogs thereof wherein the hybridization of the probe and the mRNA for human methenyltetrahydrofolate synthetase is substantially preserved. The present invention also relates to a polyclonal antibody or an antigen-binding fragment thereof for the detection of the mRNA for human methenyltetrahydrofolate synthetase, wherein said antibody is raised against a protein segment aa100-112 H-Phe-Asp-Lys-His-Gly-Asn-Arg-Leu-Gly-Arg-Gly-Lys-OH, Seq. Id. No. 2 of human methenyltetrahydrofolate synthetase.