METHOD FOR PRODUCING OLIGOSACCHARIDES

摘要

The present invention provides a method of producing oligosaccharides by the reaction between lactose and .beta.-galactosidase from Aspergillus oryzae in which an concentration of lactose in an enzyme reaction mixture is between 50 and 90% (w/v) and a reaction temperature is within the range of 55.degree.C to the temperature at which the .beta.-galactosidase in the reaction mixture is inactivated. This invention therefore makes it possible to obtain the sugar mixture containing high-purity oligosaccharides, small amounts of unreacted lactose and by produced monosaccharides.