| 摘要 |
PURPOSE:To efficiently produce a cyclic inuloligosaccharide from beta-2,1-fructose oligosaccharide, inulin, etc., by using a culture solution obtained by culture of a new protein and a transformant containing a gene encoding the protein or a separated cell. CONSTITUTION:A new water-soluble enzyme protein having cycloinulosaccharide fructanotransferase activity and about 120,000 molecular weight by SDS- polyacrylamide gel electrophoresis and gel filtration method is separated from a bacteria belonging to the genus Bacillus and purified. A base sequence encoding the protein is determined and the protein is produced by using a transformant transformed by a manifestation vector having the base sequence. A culture solution obtained by culturing the transformant, a separated cell, etc., is treated with beta-2,1-fructose oligosaccharide, inulin, etc., to produce the cyclic inulo-oligosaccharide. |
