摘要 |
PURPOSE:To produce formic acid easily and economically by biochemical means, by reacting carbon monoxide with water in the presence of a cultured liquid, cultured cells or treated cells of microorganism belonging to Methanosarcina genus. CONSTITUTION:A microbial strain belonging to Methanosarcina genus and capable of producing methane, e.g. Methanosarcina barkeri, etc., is cultured under anaerobic condition, and the cultured liquid, microbial cells separated from the cultured liquid, or treated microbial cells are collected. The obtained cells or treated cells, etc., may be used in immobilized state. Carbon monoxide is made to react with water under anaerobic condition in the presence of the microbial cells in a reaction liquid added with an electron acceptor such as methyl viologen to obtain formic acid. The produced formic acid is separated and purified by conventional extraction process. |