MODIFIED DNA VIRUS VECTORS AND USES THEREFOR

摘要

Methods and compositions are described for production of desired peptides or proteins in vitro or in vivo. A system for rapidly inserting exogenous DNA encoding a selected protein or peptide into a DNA virus is provided. One embodiment of the invention includes a modified HSV-1 virus, which comprises at least one restriction endonuclease site foreign to wild-type HSV and a universal cloning cassette containing the exogenous DNA flanked by the foreign restriction enzyme site and a restriction enzyme site native to the HSV-1 virus.