A METHOD FOR SYNTHESIS OF POLYSACCHARIDES

摘要

Methods of synthesizing cellulose I in vitro are disclosed. One method comprises contacting an activated saccharide substrate with an endoglucanase in an appropriate organic solvent/buffer ratio. The invention also encompasses a purified endoglucanase. A second method comprises contacting a nucleotide sugar with a purified glycosyl transferase in an appropriate buffer medium to insure polymerization and crystallization of parallel chains from the enzyme/micelle complex to form cellulose I.