| 摘要 |
Antisense oligodeoxynucleotides to fibrogenic cytokines such as mRNA-TGF-.beta., mRNA-TNF-.alpha., mRNA-PDGF, are effective in prohibiting a scar is described. For an example, anti sense oligodeoxynucleotide to TGF-.beta., 5'-CCGAGAGGGGAACAGGGC-3' is synthesized in an automatic DNA synthesizer(Pharmacia, LKB) at room temperature for 2.5 hr, which is combined with linker GGGCC, transfused on Apa I terminal site of pBluescript to give a recombined expression plasmid, which is cultured in Eschericha coli. HB101. The transformed E coli HB101 is fermented in LG media at 37 deg.C for 16 hr to express anti sense oligodeoxynucleotides. |
