发明名称 |
Improved sol capture immunoassay procedure |
摘要 |
An immunoassay procedure for detection of analytes in urine wherein an immunological reaction is conducted in an aqueous phase containing the urine and a filterable immunocomposite containing an inherently colored gold sol particle is formed if the assay is positive. The colored, gold sol particle containing immunocomposite is collected for direct visual observation on a filter element. False positive readings are minimized by (1) removing contaminants from the urine to prevent clogging of the filter element and non-specific binding of unreacted reactants thereto by contacting the urine with a porous contact element having a detergent material dispersed on its external and interstitial surfaces; (2) preventing non-specific binding of unreacted immunoreactive substances to the filter element by blocking the latter with polyvinyl pyrrolidone; and/or (3) including a green dye material in the aqueous reaction phase to improve the optical distinction after washing the collection filter between negative and positive results on the filter. |
申请公布号 |
AU656451(B2) |
申请公布日期 |
1995.02.02 |
申请号 |
AU19930031803 |
申请日期 |
1993.01.14 |
申请人 |
HYGEIA SCIENCES, INC |
发明人 |
CHARLES C. DITLOW;L. EDWARD CANNON;FRANCIS X. COLE;GENE A. DAVIS;ERIC C. SIGILLO;ALICIA G. DANTI |
分类号 |
G01N33/53;G01N33/543;G01N33/58;(IPC1-7):G01N33/553;G01N33/76 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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