发明名称 Enzyme conjugate prepared with insoluble nonoparticle
摘要 The invention relates to a reagent consisting of at least one enzyme and at least one other substance, which are covalently or noncovalently bound to a particle, which is smaller than or is equal to 1000 Angstrom in diameter. The invention also relates to method and use of the reagent for determination or studies of a cell or a virus or another component in a sample. According to the invention, these determinations are made with for example some ELISA-technique (competitive, sandwich, etc), employing the reagent preferably in the form of a suspension in buffered water, instead of and in the same way as described for soluble enzyme conjugates. The substance can be antibody, a lectin, avidin or an antigen, the enzyme can be for example peroxidase, alcaline phosphatase, and the particle can be an inorganic or an organic polymeric compound or a combination thereof, as, for example, tresyl-activated glycerylpropyl-silica.
申请公布号 US5405752(A) 申请公布日期 1995.04.11
申请号 US19940185213 申请日期 1994.01.14
申请人 NILSSON, KURT G. I. 发明人 NILSSON, KURT G. I.
分类号 G01N33/543;G01N33/58;(IPC1-7):G01N33/53 主分类号 G01N33/543
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