| 发明名称 |
SELECTION MARKER GENE FREE RECOMBINANT STRAINS: A METHOD FOROBTAINING THEM AND THE USE OF THESE STRAINS |
| 摘要 |
The present invention discloses a selection marker free system which can be used to introduce genetic modifications in bacteria, yeasts and fungi. The system can be employed to introduce or delete desired genes or DNA fragments in the genome of the indicated host species without leaving any undesired DNA i.e. the selection marker used for selection of transformants or other DNA used for cloning. In this way strains have been developed containing only desired genes introduced at desired chromosomal sites. Similarly, desired DNA fragments have been deleted or replaced at desired sites.
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| 申请公布号 |
CA2128589(A1) |
申请公布日期 |
1995.01.24 |
| 申请号 |
CA19942128589 |
申请日期 |
1994.07.21 |
| 申请人 |
GIST-BROCADES N.V. |
发明人 |
SELTEN, GERARDUS C. M.;SWINKELS, BART W.;VAN GORCOM, ROBERTUS F. M. |
| 分类号 |
C12N15/09;C12N;C12N1/00;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N15/00;C12N15/01;C12N15/31;C12N15/52;C12N15/63;C12N15/64;C12N15/65;C12N15/70;C12N15/73;C12N15/74;C12N15/75;C12N15/80;C12N15/81;C12R1/10;C12R1/125;C12R1/19;C12R1/645;C12R1/66;C12R1/685;C12R1/80;C12R1/865;C12R1/885;(IPC1-7):C12N15/80 |
主分类号 |
C12N15/09 |
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