发明名称 |
Ubiquitin carrier enzyme E2-F1, purification, production, and use |
摘要 |
A method for isolating and purifying novel species of E2 ubiquitin-carrier protein, designated E2-F1, is disclosed. A method for preparing enzymatically active fragments of E2-F1 enzyme is also disclosed. The use of purified E2-F1 to produce antibodies is also disclosed. The use of such E2-F1-specific antibodies to detect the presence of E2-F1 in a biological sample, and to inhibit protein degradation are also disclosed. Recombinant DNA molecules which code for E2-F1, and recombinant hosts and vectors which contain E2-F1 coding sequences are also disclosed. The use of such recombinant hosts and vectors to produce E2-F1 protein is also disclosed. The use of purified E2-F1 to identify and to isolate E3 enzyme is also disclosed. Methods for screening substances for the ability to inhibit E2-F1 enzyme activity are also disclosed.
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申请公布号 |
US5384255(A) |
申请公布日期 |
1995.01.24 |
申请号 |
US19930080073 |
申请日期 |
1993.06.21 |
申请人 |
RAPPAPORT FAMILY INSTITUTE FOR RESEARCH IN THE MEDICAL SCIENCES |
发明人 |
CIECHANOVER, AARON J.;BLUMENFELD, NAVA;GONEN, HEDVA |
分类号 |
C12N15/09;C12N9/00;C12N9/64;C12N11/02;C12N15/54;C12P21/08;C12R1/19;(IPC1-7):C12N9/10 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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