发明名称 |
METHOD FOR SAFETY EVALUATION OF CHEMICAL COMPOUND USING RECOMBINANT YEAST EXPRESSING HUMAN CYTOCHROME P450 |
摘要 |
of the disclosure: There is disclosed a method for evaluation of the safety of a chemical compound, which includes the steps of: (a) reacting a chemical compound with recombinant yeast cells expressing, or in other words producing, human cytochrome P450 molecular species P450 1A2, P450 2C9, P450 2E1 and P450 3A4 together with a yeast NADPH-P450 reductase, which may be in the form of a fused enzyme with each of said human cytochrome P450 molecular species, or with the cell free extracts of the yeast cells; and (b) analyzing the resulting metabolite to determine the safety of the compound. According to this method, it can be determined whether a test compound will be converted into a carcinogenic or mutagenic form through the metabolism in the human liver, and whether the test compound or its metabolite has mutagenicity.
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申请公布号 |
CA2128399(A1) |
申请公布日期 |
1995.01.21 |
申请号 |
CA19942128399 |
申请日期 |
1994.07.19 |
申请人 |
HAYASHI, KOJI;SAKAKI, TOSHIYUKI;YABUSAKI, YOSHIYASU;KOMAI, KOICHIRO;KANEKO, HIDEO;NAKATSUKA, IWAO |
发明人 |
HAYASHI, KOJI;SAKAKI, TOSHIYUKI;YABUSAKI, YOSHIYASU;KOMAI, KOICHIRO;KANEKO, HIDEO;NAKATSUKA, IWAO |
分类号 |
C12N9/02;C12N15/53;C12Q1/02;(IPC1-7):C12N15/62;C12Q1/26;C12Q1/68 |
主分类号 |
C12N9/02 |
代理机构 |
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主权项 |
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地址 |
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