A DNA CONSTRUCT ENCODING THE YAP3 SIGNAL PEPTIDE

摘要

A DNA construct comprising the following sequence: 51-P-SP-(LP)n-PS-HP-31 wherein P is promoter sequence, SP is a DNA sequence encoding the yeast aspartic protease 3 (YAP3) signal peptide, LP is a DNA sequence encoding a leader peptide, n is 0 or 1. PS is a DNA sequence encoding a peptide defining a yeast processing site, and HP is a DNA sequence encoding a polypeptide which is heterologous to a selected host organism. The YAP3 signal peptide provides efficient secretion of heterologous proteins in yeast.