| 摘要 |
An automated method for the assay of total bilirubin in bodily fluids, a solubilizing reagent for bilirubin and a diagnostic test kit for assay of total bilirubin. A first spectrophotometric measurement of the absorbance of bilirubin is made using two wavelengths, one of which is between about 450-480 nm and the other is between about 600-660 nm. The absorbance of bilirubin is destroyed by an oxidizing agent, and a second spectrophotometric measurement is made at the same two wavelengths. The assay of total bilirubin is calculated from the absorbance readings, along with the absorbance readings obtained from a test standard and a reagent blank. |
