发明名称 |
A SYNTHETIC GENE FOR D4 DOPAMINE RECEPTORS |
摘要 |
A modified gene coding for the human D4 dopamine receptor has been synthesized by chemo-enzymatic methods. The nucleotide sequence of the D4 dopamine receptor gene was changed to reduce the G+C content and to eliminate intronic sequences, while maintaining the published amino acid sequence. Using gene splicing by overlap extension and PCR amplification of long oligonucleotides (> 200 bases), 3 synthetic fragments of about 400 base pairs each were amplified, from which the full length gene was assembled. Stable expression of this gene has been achieved in CHO-K1 cells, using an inducible expression system, and in HEK293 cells.
|
申请公布号 |
CA2163432(A1) |
申请公布日期 |
1995.01.12 |
申请号 |
CA19942163432 |
申请日期 |
1994.06.01 |
申请人 |
PHARMACIA & UPJOHN COMPANY |
发明人 |
HUFF, RITA M.;CHIO, CHRISTOPHER L. |
分类号 |
C12N5/00;C07K14/705;C12N5/10;C12N15/09;C12N15/12;C12R1/91;G01N33/566;(IPC1-7):C12N15/12;C12Q1/02 |
主分类号 |
C12N5/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|