发明名称 |
A SYNTHETIC GENE FOR D4 DOPAMINE RECEPTORS |
摘要 |
A modified gene coding for the human D4 dopamine receptor has been synthesized by chemo-enzymatic methods. The nucleotide sequence of the D4 dopamine receptor gene was changed to reduce the G+C content and to eliminate intronic sequences, while maintaining the published amino acid sequence. Using gene splicing by overlap extension and PCR amplification of long oligonucleotides (> 200 bases), 3 synthetic fragments of about 400 base pairs each were amplified, from which the full length gene was assembled. Stable expression of this gene has been achieved in CHO-K1 cells, using an inducible expression system, and in HEK293 cells.
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申请公布号 |
WO9501435(A1) |
申请公布日期 |
1995.01.12 |
申请号 |
WO1994US05986 |
申请日期 |
1994.06.01 |
申请人 |
THE UPJOHN COMPANY;CHIO, CHRISTOPHER;HUFF, RITA, M. |
发明人 |
CHIO, CHRISTOPHER;HUFF, RITA, M. |
分类号 |
C12N5/00;C07K14/705;C12N5/10;C12N15/09;C12N15/12;C12R1/91;G01N33/566;(IPC1-7):C12N15/12 |
主分类号 |
C12N5/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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