| 摘要 |
PURPOSE:To allow complicated separation of a multicomponent system, e.g. optical resolution of amino acids, by injecting a sample only once. CONSTITUTION:In a liquid chromatography where first and second columns are coupled, a plurality of empty columns 15 and a plurality of concentrated columns 21 are coupled between the first and second columns 7, 23. A sample S is fed to the first column 7 using a first eluate composed of a mixture solvent of a rich solvent 2 and a first lean solvent 4 thus separating the components roughly. Each fraction eluted from the first column 7 is sampled by each empty column 15 and then the fraction is eluted from each empty column 15 by means of the first eluate. Subsequently, a second lean solvent 4 is added thereto before it is introduced to each concentrated column 21. The components of each fraction are then captured by respective concentrated columns 21 and subsequently eluted using a second eluate 18 before it is fed to the second column 23 to be separated. |
