NUCLEIC ACIDS OF A NOVEL HANTAVIRUS AND REAGENTS FOR DETECTION AND PREVENTION OF INFECTION

摘要

The present invention relates to the discovery and isolation of a novel Hantavirus designated the Four Corners Virus. In particular, the present invention relates to nucleic acids of the newly discovered virus and to nucleic acid reagents (primers and probes), purified polypeptides and antibodies for use in methods of detection and prevention of infection by the virus. A vaccine or purified immunogenic polypeptides of the Four Corners Virus in a pharmaceutically acceptable carrier is provided. A vector comprising the nucleic acids of the invention is provided. A method of detecting the presence of a Hantavirus in a subject comprising contacting an antibody-containing sample from the subject with a purified polypeptide of the invention and detecting the reaction of the polypeptide and the antibody is provided. A method of detecting the presence of the Four Corners Virus is provided comprising reverse transcribing viral RNA to synthesize a complementary DNA sequence followed by amplifying the DNA using primers which are selective for the Four Corners Virus and detecting the presence of amplification, thereby indicating presence of the Four Corners Virus in the sample.