发明名称 |
METHOD FOR ASSESSING MICROORGANISM VIABILITY |
摘要 |
A method for assessing microorganism viability, including: (a) loading the microorganisms with a fluorescent substance, and (b) measuring the brightness of the intracellular fluorescence by flow cytometry. The method is characterised in that it includes the steps of: (i) incubating said microorganisms for 5-20 minutes with the marking substance in a concentration of 1-300 mu M and in the presence of a marking buffer at a pH of 4-8, to give microorganisms loaded with a fluorescent substance (loading step (a)); (ii) removing all of the marking substance not incorporated into the microorganisms; (iii) adding a source of energy prior to step (b); and (iv) using flow cytometry to measure the time required for half of the intracellular fluorescence of the microorganisms to disappear, within a predetermined brightness range, at a pH of 4-8 (outflow buffer) and at a temperature of 10-40 DEG C, preferably 20-40 DEG C.
|
申请公布号 |
WO9500660(A1) |
申请公布日期 |
1995.01.05 |
申请号 |
WO1994FR00770 |
申请日期 |
1994.06.27 |
申请人 |
CHEMUNEX;BREEUWER, PIETER;DROCOURT, JEAN-LOUIS |
发明人 |
BREEUWER, PIETER;DROCOURT, JEAN-LOUIS |
分类号 |
C12M1/34;C12Q1/02;C12Q1/04;(IPC1-7):C12Q1/02 |
主分类号 |
C12M1/34 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|