A NOVEL FAMILY OF POLLEN PROTEINASES, METHODS OF USE THEREOF AND COMPOSITIONS DERIVED THEREFROM

摘要

A novel family of proteins has been identified. The proteins are derived from allergenic pollens and exhibit serine-proteinase-like activity. The proteins have a molecular weight of about 85-95 kD as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis; and a molecular weight of about 67 kD as determined by Fast Protein Liquid Chromatography. The proteins are resistant to inhibition by alpha -2-macroglobulin, alpha -1-proteinase inhibitor, trypsin inhibitors and are sensitive to inhibition by phenyl methane sulfonyl fluoride, difluorophenol, benzamidine, antipain, leupeptin and tosyl-L-lysine chloromethyl ketone.