发明名称 |
A METHODOLOGY FOR SELECTING SUPERAGONISTS, ANTAGONISTS AND SUPERANTAGONISTS OF HORMONES WHOSE RECEPTOR COMPLEX INCLUDES GP 130 |
摘要 |
It has been found that the ligands of the group of cytokins similar to Interleukin 6 (IL-6), that is Oncostatin M (OSM), Leukemia Inhibitory Factor (LIF), Ciliary Neurotrophic Factor (CNTF) and Interleukin 11 (IL-11), induce formation of a receptor complex of which the membrane molecule gp 130 is a part. It is thus possible to formulate the hypothesis that two different surfaces of the protein (in this class of hormones), known as site 1 and site 2, bind to two different molecules: site 1 to the specific receptor and site 2 to gp 130. The invention allows the identification of these sites and the isolation of variants having, with respect to the wild hormone, a greater affinity for the specific receptor (superagonists and superantagonists) or affinity for gp 130 reduced or abolished (antagonists and superantagonists). The figure shows the construct pHen DELTA hIL-6 and the production of phasmidic particles that can be used to select agonists of Interleukin 6. |
申请公布号 |
WO9500852(A2) |
申请公布日期 |
1995.01.05 |
申请号 |
WO1994IT00095 |
申请日期 |
1994.06.23 |
申请人 |
ISTITUTO DI RICERCHE DI BIOLOGIA MOLECOLARE P. ANG;SAVINO, ROCCO;LAHM, ARMIN;CILIBERTO, GENNARO |
发明人 |
SAVINO, ROCCO;LAHM, ARMIN;CILIBERTO, GENNARO |
分类号 |
G01N33/53;C07K14/475;C07K14/52;C07K14/54;C07K14/705;C07K19/00;C12N15/12;C12N15/18;G01N33/566;G01N33/68;G01N33/74 |
主分类号 |
G01N33/53 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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