| 摘要 |
The present invention relates to a mammalian expression system comprising a DNA sequence encoding a polypeptide having the amino acid sequence shown in SEQ ID NO:2 or a variant thereof which has a biological activity of human EC-SOD. The mammalian expression system is preferably expressed in a non-human mammal selected from the group consisting of rabbits, mice, rats, goats, sheep, pigs, lama, camels and bovine species. The invention includes expression systems, wherein the variant has a reduced heparin affinity as compared to human EC-SOD type C such as the variants T216, T215, T213, T209, SAT216, SRT216, SA219, SA220 and SA211-213, and expression systems wherein the variant has an increased heparin affinity as compared to human EC-SOD type C such as the variants SA216, SA216/218 and SA216/220. Within the scope of the invention is also DNA fragments, hybrid genes, expression vectors, cells, methods for producing a transgenic non-human mammal capable of expressing EC-SOD as defined above, and non-human mammals expressing EC-SOD.
|
