| 摘要 |
<p>The present invention relates to an assay for the formation of multi-protein complexes comprising the steps of: i) reacting a first protein of a multi-protein complex with an immobilised first antibody specific therefor which does not interfere with complex formation; ii) optionally adding further proteins which form part of the multi-protein complex; iii) optionally adding a test substance; iv) adding the remaining protein(s) required for formation of the multi-protein complex; v) adding a labelled second antibody specific to a protein added in step (iv); and vi) detecting and optionally determining the amount of the second antibody immobilised as an indication of multi-protein complex formation. Such an assay can be used to determine whether or to what degree a naturally produced multi-protein complex is formed by an individual. In this way any malfunction in formation of a multi-protein complex, for example due to a genetic disorder or physiological disturbance can be ascertained.</p> |
