发明名称 |
Fremgangsmåte for fremstilling av rekonstituert lipoprotein |
摘要 |
Industrial prodn. of a compsn. (A), contg. reconstituted lipoprotein particles (LPP), comprises first mixing an aq. apolipoprotein (APL) soln. of protein content 1-40 g/l with an aq. lipid(L)-detergent (D) soln., at mole ratio L:D 1:0.5-4 and wt. ratio APL:L 1:1.5-5.The resulting mixt. was incubated at a temp. within 10 degrees C of the phase transition temp. of L in water, then the detergent removed (by exclusion according to mol. size or by using an adsorbent) so that LPP of dia. 5-50 nm and mol.wt. 0.05-1 MD are formed. Without further purificn. steps, over 95% of added protein and over 90% of total L is bound in LPP.Opt. the resulting liq. prod. is lyophilised in presence of a carbohydrate stabiliser.(A) contains 18-22 g/l protein and, at 18-22 degrees C, has turbidity below 40 NTV. |
申请公布号 |
NO945101(D0) |
申请公布日期 |
1994.12.30 |
申请号 |
NO19940005101 |
申请日期 |
1994.12.30 |
申请人 |
ROTKREUZSTIFTUNG ZENTRALLABORATORIUM BLUTSPENDEDIENST SRK |
发明人 |
LERCH, PETER;HODLER, GERHARD;FORTSCH, VRENI |
分类号 |
A61K38/00;A61K9/127;A61P3/06;C07K1/22;C07K14/775;(IPC1-7):C07K |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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