| 摘要 |
Apparatus for studying the interaction of first and second molecules in a test solution containing fluorescently labeled molecules in addition to the first and second molecules, with the first molecules and the fluorescently labeled molecules being capable of binding with the second molecules. The apparatus comprises a flow channel having opposite, spaced apart walls, with at least one of the walls or a portion thereof being translucent or transparent. A porous matrix is retained in a fixed position between the walls of the flow channel and in direct contact with a translucent or transparent portion of the walls of the flow channel. A test solution flows through the flow channel and around the porous matrix so as to be in contact with the porous matrix. The porous matrix comprises a material that is translucent or transparent and has pores or openings therein which are of a microscopic size such that the unbound fluorescently labeled molecules can permeate the porous matrix, while the second molecules and fluorescently labeled molecules bound to the second molecules cannot permeate the porous matrix. The porous matrix is substantially inert and does not react chemically with any of the molecules contained in the solution. Fluorescence emitted by the fluorescently labeled molecules permeating the porous matrix is detected through the translucent or transparent portion of the walls of the flow channel.
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