| 摘要 |
<p>A liquid flow cytometer for counting and classification of particles such as biological cells and other microscopic particles in liquids, where the cytometer comprises an optical system for irradiation and detection in a metering zone (7), thus enabling optical dispersion and fluorescence caused by the particles to be simultaneously detected when the particles pass the metering zone. The optical system comprises in a first beam path (S1) a mirror reflector (9) with its focal point in the metering zone (7), a quarter-wave plate (8) between the metering zone (7) and the mirror reflector (9), a lens (6) with its focal point in the metering zone (7) and located on the opposite side of it together with a dichroic mirror (5) behind the lens and provided in such a manner that it gives reflection to a second beam path (S2). In the second beam path (S2) a beam splitter (4) is provided between the dichroic mirror (9) and a known per se light source (1), and produces a third beam path (S3). The cytometer comprises a firt detector (13) provided in the first beam path (S1) behind the dichroic mirror (5) and arranged for the detection of fluorescence emitted by the particles in the metering zone (7), together with a second detector (16) provided in the third beam path (S3) and arranged for the detection of optical dispersion caused by the particles in the metering zone (7).</p> |
