ACTIVE HETEROCONJUGATES OF CELLOBIOHYDROLASE AND BETA-GLUCOSIDASE

摘要

Active heterodimers are described which are prepared by connecting molecules of beta-glucosidase and cellobiohydrolase with a bifunctional reagent (i.e., a crosslinking agent) or by genetic fusion of the two polypeptide chains. The ability of the beta-glucosidase to catalyze hydrolysis of cellobiose to glucose, and the ability of cellobiohydrolase to bind to crystalline cellulose and to catalyze the cleavage of cellobiosyl residues from the non-reducing ends of the cellulose chains, are all retained in the combined molecule.