发明名称 |
Avidin-and biotin immobilized reagents and methods of use |
摘要 |
Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.
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申请公布号 |
US5374516(A) |
申请公布日期 |
1994.12.20 |
申请号 |
US19890315086 |
申请日期 |
1989.02.24 |
申请人 |
EASTMAN KODAK COMPANY |
发明人 |
SUTTON, RICHARD C.;BURDICK, BRENT A.;DANIELSON, SUSAN J.;WARREN, III, HAROLD C.;SNYDER, BRIAN A.;MCCLUNE, GREGORY J.;WU, ANNIE L. |
分类号 |
G01N33/543;G01N33/545;(IPC1-7):G01N33/545;G01N33/546 |
主分类号 |
G01N33/543 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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