发明名称 |
Process for stabilizing 1-methylhydantoinase, use and method of it for determining an analyte and agents suitable for this |
摘要 |
The invention concerns a process for stabilizing the enzyme 1-methylhydantoinase (NMHase) which is characterized in that the NMHase is treated with divalent metal ions, a nucleoside triphosphate and a complexing agent which complexes these divalent metal ions. In addition the invention concerns the use of an enzyme stabilized in this way in a method for the determination of an analyte in which 1-methylhydantoin is converted, as well as a corresponding reagent.
|
申请公布号 |
US5374546(A) |
申请公布日期 |
1994.12.20 |
申请号 |
US19920822176 |
申请日期 |
1992.01.16 |
申请人 |
BOEHRINGER MANNHEIM GMBH |
发明人 |
NAGEL, ROLF;DENEKE, ULFERT;MISTELE, JUERGEN |
分类号 |
C12N9/86;C12N9/96;C12Q1/26;C12Q1/28;C12Q1/34;(IPC1-7):C12N9/96;C12N9/78 |
主分类号 |
C12N9/86 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|