| 摘要 |
One method of determining the HCV genotype of a HCV strain is provided which involves subjecting the HCV strain to two stage PCR, wherein the first stage PCR involves utilizing antisense primer #186 (SEQ ID NO:5) and at least one of sense primers #256 (SEQ ID NO:4) and #256V (SEQ ID NO:1) for first stage amplification and wherein the second stage PCR involves utilizing at least one of sense primers #104 (SEQ ID NO:6) and #104V (SEQ ID NO:2) and at least one of antisense primers #296 (SEQ ID NO:7), #133 (SEQ ID NO:8), #134 (SEQ ID NO:9), #135 (SEQ ID NO:10), and #339 (SEQ ID NO:3) for the second stage amplification; the method further involves comparing the product of the second stage PCR with HCV genotypes I, II, III, IV, and V. The method may further involve synthesizing cDNA from viral RNA of the HCV strain and/or measuring the molecular weight of the product of the second stage PCR.
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