NUCLEIC ACID ANALOGUE ASSAY PROCEDURES

摘要

An assay procedure to quantify the amount of starting template in a nucleic acid amplification procedure such as PCR involves trying the amplification in the presence of varying amounts of a nucleic acid analogue which in sufficient quantity will inhibit said amplification procedure by selective interaction with a nucleic acid species present, e.g. a PNA as described in WO-02-20703. The amount of template is given by the amount of nucleic acid analogue critical to cause failure of said amplification procedure.