| 摘要 |
<p>The method of the invention comprises the implementation of axenic conditions, with the utilisation of a liquid monophase culture medium. In order to obtain amastigote forms, said medium is buffered to a pH from 5.5 to 6.5 and has an osmolarity of at least 400 milli osmoles/kg, and in particular from 400 to 550 milli osmoles/kg of liquid. In order to obtain promastigote forms, said medium is buffered to a pH from 7 to 7.5 and has an osmolarity of at least 300 milli osmoles/kg of liquid. Said method provides for the adaptation in the culture in vitro of differents stages of tissue parasites such as leishmanies and T. cruzi or the hematoprotozoans.</p> |
